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Antioxidant effect of vitamin C on porcine oocytes maturated in vitro

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dc.contributor.author Tulcan, Camelia
dc.contributor.author Cernescu, Horia
dc.contributor.author Huțu, Ioan
dc.contributor.author Boldura, Oana
dc.contributor.author Otavă, Gabriel
dc.contributor.author Furcă, Oana
dc.contributor.author Mircu, Călin
dc.contributor.author Marc, Simona
dc.date.accessioned 2021-04-13T09:02:57Z
dc.date.available 2021-04-13T09:02:57Z
dc.date.issued 2019
dc.identifier.citation Tulcan, Camelia, Horia Cernescu, Ioan Huțu, Oana Boldura, Gabriel Otavă, Oana Furcă, Călin Mircu, Simona Marc . 2020. " Antioxidant effect of vitamin C on porcine oocytes maturated in vitro". Lucrări Științifice USAMV - Iași Seria Medicină Veterinară 62 (3): 205-210. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/752
dc.description.abstract Cells are vulnerable to oxidative stress during in vitro culture systems. The objective of the present study was to determine the effect of vitamin C addition in in vitro culture media on porcine oocytes maturation rate based on morphological changes. Porcine COC’s were matured according to their morphological class (class I, II and III) in two groups: control (M) and supplemented with vitamin C (0.5 mM, C) in TCM 199 HEPES (M2520) modification media with hormones (0.88UI/ml FSH, F8174) at 38.50C in 5% CO2 humidified air atmosphere for 44h. The rates of oocytes with cumulus cells expansion were higher with addition of vitamin C as compared to control group, with 7.83% (C1), 70.59% (C2) and 6.04% (C3). It could be concluded from this preliminary study that addition of vitamin C in in vitro maturation medium has a beneficial effect on porcine oocytes especially in C2 group. en_US
dc.language.iso en en_US
dc.publisher ”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iasi en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject antioxidants en_US
dc.subject porcine oocyte en_US
dc.subject in vitro maturation en_US
dc.title Antioxidant effect of vitamin C on porcine oocytes maturated in vitro en_US
dc.type Article en_US
dc.author.affiliation Camelia Tulcan, Horia Cernescu, Ioan Huțu, Oana Boldura, Gabriel Otavă, Oana Furcă, Călin Mircu, Simona Marc,University of Agricultural Sciences and Veterinary Medicine of Banat “The King Michael I of Romania”, Faculty of Veterinary Medicine, Timisoara, Romania
dc.publicationName Lucrări Științifice USAMV - Iași, Seria Medicină Veterinară
dc.volume 62
dc.issue 3
dc.publicationDate 2019
dc.startingPage 205
dc.endingPage 210
dc.identifier.eissn 2393-4603


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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)