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Identification of coronaviral antibodies and coronavirus - specific antibody complexes in ascites fluid of cats diagnosticated with feline infectious peritonitis

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dc.contributor.author Horhogea, Cristina-Elena
dc.contributor.author Laiu, Ivona
dc.contributor.author Le Poder, Sophie
dc.contributor.author Carp Cărare, Mihai
dc.contributor.author Rîmbu, Cristina-Mihaela
dc.contributor.author Carp Cărare, Cătălin
dc.date.accessioned 2022-05-04T11:06:18Z
dc.date.available 2022-05-04T11:06:18Z
dc.date.issued 2010-11-19
dc.identifier.citation Horhogea, Cristina, Ivona Laiu, Sophie Le Poder, M. Carp-Cărare, Cristina Rîmbu, C. Carp-Cărare. 2011. “Identification of coronaviral antibodies and coronavirus - specific antibody complexes in ascites fluid of cats diagnosticated with feline infectious peritonitis”. Cercetări Agronomice în Moldova 44 (2): 87-93. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/2393
dc.description.abstract Feline infectious peritonitis (FIP) is an infectious lethal cat diseases, prodused by a mutant feline coronavirus (feline infectious peritonitis virus), which is manifested in two clinical forms. Dry form may go unnoticed or be confused with other diseases. Wet form, however, evolve with ascites fluid accumulation, its appearance being correlated with end-stage of the disease. Research has pursued the efficiency of direct immunofluorescence test for the identification of coronavirus - anticoronavirus antibody complexes in ascites fluid. Nine ascites fluid samples, obtained from cats aged between 1,7 months and 13 years, diagnosed with feline infectious peritonitis, were analyzed. The antibody titrers were assessed using indirect immunofluorescence on pig kidney (PK) cell cultures infected with TGEV, on three samples, titres ranging from 1/25 and 1/625. All nine ascites fluid samples tested by direct immunofluorescence for detection of coronavirus specific antibodies complexes were positive. In images obtained with UV in microscopy, fluorescence being seen in the macrophages under the form of a ring arranged on the periphery of the cell membrane and fluorescence localized intracellularly, probably internalized immune complexes. The results lead us to recommend the use of this test for FIP rapid diagnostic. en_US
dc.language.iso en en_US
dc.publisher "Ion Ionescu de la Brad" University of Agricultural Sciences and Veterinary Medicine, Iași en_US
dc.subject Coronavirus; Peritonitis; Immunofluorescence; Ascites; Complex en_US
dc.subject coronavirus en_US
dc.subject peritonitis en_US
dc.subject immunofluorescence en_US
dc.subject ascites en_US
dc.subject complex en_US
dc.title Identification of coronaviral antibodies and coronavirus - specific antibody complexes in ascites fluid of cats diagnosticated with feline infectious peritonitis en_US
dc.type Article en_US
dc.author.affiliation Cristina Horhogea, Ivona Laiu, M. Carp-Cărare, Cristina Rîmbu, C. Carp-Cărare, University of Agricultural Sciences and Veterinary Medicine Iaşi, Romania
dc.author.affiliation Sophie Le Poder, Ecole Nationale Vétérinaire d’Alfort , France
dc.publicationName Cercetări Agronomice în Moldova
dc.volume 44
dc.issue 2
dc.publicationDate 2011
dc.startingPage 87
dc.endingPage 93
dc.identifier.eissn 2067-1865


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