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Detection of Orf virus and papillomavirus out of samples from goats and cattle which were gathered by multiplex PCR Romania

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dc.contributor.author Strugaru, Oana-Raluca
dc.contributor.author Velescu, Elena
dc.contributor.author Perianu, Tudor
dc.contributor.author Bocăneți, Florentina
dc.contributor.author Scagliarini, Alessandra
dc.date.accessioned 2021-10-20T10:01:07Z
dc.date.available 2021-10-20T10:01:07Z
dc.date.issued 2012-02-01
dc.identifier.citation Strugaru, Oana Raluca, Elena Velescu, T. Perianu, Florentina Bocăneţi, Alessandra Scagliarini. 2012. ”Detection of Orf virus and papillomavirus out of samples from goats and cattle which were gathered by multiplex PCR Romania”. Cercetări Agronomice în Moldova 45 (2): 85-88. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1946
dc.description.abstract The lesions which appear on the skin and are caused by parapoxvirus, herpesviruses and papillomavirus, depending on they’re gravity, can produce large economic losses. The identification of viral species with different laboratory methods can facilitate making a correct diagnostic in identifying the infectious entity. The most common encountered injuries , especially on goats and kids, have an oral location, breasts, eyes and genital and podal. On the other side on cattles infected with papillomavirus the lesions are multiple and spread all over the body. To identify the etiologic agent from goats and cattle with skin lesions, tissue samples were collected and scabs. The virus identification was made with molecural biology techniques. The PCR method revealed the viral DNA, thus identifying a portion of the B2L preserved gene which is then amplified using primers PPP1-PPP4. For papillomavirus detection, primers FAP59 and FAP 64 were used to conserve the L1 human gene, primers that can help detect other types of papillomavirus from other species. The PCR results showed that lesions from goats were made by Orf virus, a virus that belongs to the papillomavirus gene, as for cattles the bovine papillomavirus. Studies have shown that PCR is a rapid laboratory method which can be used to identify the viral agent that causes severe skin lesions. en_US
dc.language.iso en en_US
dc.publisher "Ion Ionescu de la Brad" University of Agricultural Sciences and Veterinary Medicine, Iași en_US
dc.subject Viruses en_US
dc.subject PCR en_US
dc.subject PCR en_US
dc.subject Injuries en_US
dc.title Detection of Orf virus and papillomavirus out of samples from goats and cattle which were gathered by multiplex PCR Romania en_US
dc.type Article en_US
dc.author.affiliation Oana Raluca Strugaru, Elena Velescu, T. Perianu, Florentina Bocăneţi, University of Agricultural Sciences and Veterinary Medicine “Ion Ionescu de la Brad” Iaşi, Romania
dc.author.affiliation Alessandra Scagliarini, University of Bologna - Department of Veterinary Public Health and Animal Pathology, Italy
dc.publicationName Cercetări Agronomice în Moldova
dc.volume 45
dc.issue 2
dc.publicationDate 2012
dc.startingPage 85
dc.endingPage 88
dc.identifier.eissn 2067-1865


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