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Identification and antifungal susceptibility testing of some candida Spp. strains using modern phenotypic methods

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dc.contributor.author Nadăș, George Cosmin
dc.contributor.author Chirilă, Flore
dc.contributor.author Bouari, Cosmina Maria
dc.contributor.author Nicodim Iosif, Fit
dc.date.accessioned 2021-07-12T05:28:36Z
dc.date.available 2021-07-12T05:28:36Z
dc.date.issued 2016
dc.identifier.citation Nadăș, George Cosmin, Flore Chirilă, Cosmina Maria Bouari, Nicodim Iosif Fiț. 2016. "Identification and antifungal susceptibility testing of some candida Spp. strains using modern phenotypic methods". Lucrări Științifice USAMV-Iași Seria Medicină Veterinară 59 (4): 470-473. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1701
dc.description.abstract The aim of this study was to identify Candida isolates from animals and humans using VITEK 2 card system, a modern phenotypic method testing 64 biochemical characters and the susceptibility testing using MIC method on the same Vitek 2, for 6 modern antifungals. The research was conducted during January-June 2016 in the Department of Microbiology, Faculty of Veterinary Medicine in Cluj-Napoca. Identification was made from 48 hrs isolated colonies, preparing a suspension of 1.8-2.2 optical density, and 280μl were substracted and transferred over in saline for susceptibility testing. After a period of 18-19 hours for analysis of 64 biochemical characters for each tested strain, the results were saved/printed as Laboratory report in PDF format. From the total of 20 tested strains, six were represented by Candida albicans, five strains were identified as Candida krusei, 3 were Candida tropicalis 2 Candida catenulata 2 Candida parapsilosis and other two Cryptococcus laurentii. Most identified species are susceptible to micafungin with MIC ranging from 0.06 and 0.12 depending on the species, except for Candida parapsilosis. Voriconazole was also very efficient, with MIC value of 0.12 for almost all identified species. Caspofungin efficiency is specie-related, so for C. albicans and C. tropicalis MIC is 0.25, for C. krusei is 0.5 and for C. catenulata and C. parapsilosis MIC equals 1. Flucytosine has a constant value of 1 in all identified species, except for C. krusei with MIC value of 4 or 8 - resistant strains. en_US
dc.language.iso en en_US
dc.publisher ”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Candida spp en_US
dc.subject identification en_US
dc.subject Vitek 2 en_US
dc.subject antifungals en_US
dc.title Identification and antifungal susceptibility testing of some candida Spp. strains using modern phenotypic methods en_US
dc.type Article en_US
dc.author.affiliation George Cosmin Nadăș, Flore Chirilă, Cosmina Maria Bouari, Nicodim Iosif Fiț, University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, 3-5 Calea Manastur street., 400372, Cluj-Napoca, Romania
dc.publicationName Lucrări Științifice USAMV - Iași, Seria Medicină Veterinară
dc.volume 59
dc.issue 4
dc.publicationDate 2016
dc.startingPage 470
dc.endingPage 473
dc.identifier.eissn 2393-4603


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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)