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The use of PCR for the detection of PRRS virus variants

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dc.contributor.author Fluerașu, Larion
dc.contributor.author Popa, Virgilia
dc.contributor.author Catana, Nicolae
dc.date.accessioned 2021-07-07T11:18:18Z
dc.date.available 2021-07-07T11:18:18Z
dc.date.issued 2016
dc.identifier.citation Fluerașu, Stefan, Virgilia Popa, Nicolae Catana. 2016. "The use of PCR for the detection of PRRS virus variants". Lucrări Științifice USAMV-Iași Seria Medicină Veterinară 59 (3): 340-344. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1605
dc.description.abstract The etiologic agent is an ribovirus, initially called Lelystadt virus. Subsequent research has shown that this virus has two genotypes respectively, Type 1 (European) and type 2 (American) with an considerable variability of gene sequences, with an similarity of only 50-60%. For detection of this virus have been taken lymph nodes with pathological lesions, macroscopic characteristic forPRRS syndrome, from cadavers of youth porcine, from farms where this disease evolves. Finally were intended to RT-PCR technique, an number of 12 lymph nodes. By Real Time RT-PCR version, PRRS virus was detected in only one sample (0,08%) of the 12 samples examined. By the techniques recommended by GILBERT the virus was detected in all samples (100%) and by Nested RT-PCR techniques the virus was detected in five samples (41,66%). All positive samples provided by these techniques have demonstrated that the strains belong to type 1 (European).By using the technique recommended by TRUYEN, for the detection of type 2 (US) were not detected strains belonging to this type, all samples being negative en_US
dc.language.iso en en_US
dc.publisher ”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject PRRS en_US
dc.subject lymphnode en_US
dc.subject RT-PCR en_US
dc.subject virus type 1 en_US
dc.title The use of PCR for the detection of PRRS virus variants en_US
dc.type Article en_US
dc.author.affiliation Larion Fluerașu, Nicolae Catana, Faculty of Veterinary Medicine Timişoara, Banat’s University of Agricultural Sciences and Veterinary Medicine „King Michael I of Romania” from Timișoara, Timisoara, Romania
dc.author.affiliation Virgilia Popa, SN Institutul Pasteur SA București
dc.publicationName Lucrări Științifice USAMV - Iași, Seria Medicină Veterinară
dc.volume 59
dc.issue 3
dc.publicationDate 2016
dc.startingPage 340
dc.endingPage 344
dc.identifier.eissn 2393-4603


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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)