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Comparative evaluation of three testing methods for detection of mediated resistance MBL in pseudomonas aeruginosa isolates

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dc.contributor.author Ciocan (Moțco), Oana – Alexandra
dc.contributor.author Ciftci, Alper
dc.contributor.author Carp – Cărare, Mihai
dc.contributor.author Mares, Mihai
dc.contributor.author Grecu, Mariana
dc.contributor.author Carp – Cărare, Cătălin
dc.date.accessioned 2021-07-07T06:32:05Z
dc.date.available 2021-07-07T06:32:05Z
dc.date.issued 2016
dc.identifier.citation Ciocan (Moțco), Oana – Alexandra, Alper Ciftci, Mihai Carp – Cărare, Mihai Mareș, Mariana Grecu, Cătălin Carp – Cărare, Carmen-Valentina Panzaru. 2016. "Comparative evaluation of three testing methods for detection of mediated resistance MBL in pseudomonas aeruginosa isolates". Lucrări Științifice USAMV-Iași Seria Medicină Veterinară 59 (3): 283-286. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1589
dc.description.abstract Metallo – β – lactamase (MBL) producing Pseudomonas aeruginosa have been reported to be important cause of nosocomial infections. The appearance of MBL genes and their spread among bacterial pathogens is a matter of concern with regard to the future of antimicrobial therapy. The present study was undertaken to determine which method is better to use in laboratory for detecting MBL producing P. aeruginosa. A total of 182isolates of P. aeruginosa from human and animals, 125 from human and 57 from animals, (burns, pus, urine, blood cultures, etc.), collected between 2013 and 2015 were subjected to susceptibility testing against various antibiotics by disc diffusion test according to Clinical and Laboratory Standards Institute (CLSI) guidelines 2015. Imipenem resistant isolates were selected for the detection of MBL production by E-test strips for screening MBL, double disc method (IPM and IPM+EDTA) and EDTA solution application on microcaps IPM. The positive results have been based on inhibition zone around imipenem discs impregnated with EDTA as compared to those without EDTA confirmed MBL production and for E-test strips the strains were positive those that have developed around area with EDTA solution. The double disc method (IPM and IPM+EDTA is most effective way to use in laboratory to determine early producer P. aeruginosa MBL, having 2 advantage: first is the low cost for materials (MH agar and microcaps) and the technique is very easy to be applied. en_US
dc.language.iso en en_US
dc.publisher ”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject resistance en_US
dc.subject MBL en_US
dc.subject Pseudomonas aeruginosa en_US
dc.title Comparative evaluation of three testing methods for detection of mediated resistance MBL in pseudomonas aeruginosa isolates en_US
dc.type Article en_US
dc.author.affiliation Oana – Alexandra Ciocan (Moțco), Mihai Carp – Cărare, Mihai Mareș, Mariana Grecu, Cătălin Carp – Cărare, Faculty of Veterinary Medicine, Iasi
dc.author.affiliation Alper Ciftci, University of Ondokuz Mayɪs, Faculty of Veterinary Medicine, Turkey
dc.author.affiliation Cătălin Carp – Cărare, "Grigore T. Popa" University of Medicine and Pharmacy Iasi
dc.publicationName Lucrări Științifice USAMV - Iași, Seria Medicină Veterinară
dc.volume 59
dc.issue 3
dc.publicationDate 2016
dc.startingPage 283
dc.endingPage 286
dc.identifier.eissn 2393-4603
alse.contributor.author Carmen-Valentina Panzaru


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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)