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Molecular and serological detection of cucumber mosaic virus in pepper growing areas in Isparta province, Turkey

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dc.contributor.author Çulal-Kiliç, Handan
dc.contributor.author Yardimci, Nejla
dc.date.accessioned 2021-06-30T09:58:06Z
dc.date.available 2021-06-30T09:58:06Z
dc.date.issued 2016
dc.identifier.citation Çulal-Kiliç, Handan, Nejla Yardimci. 2016. "Molecular and serological detection of cucumber mosaic virus in pepper growing areas in Isparta province, Turkey". Lucrări Ştiinţifice USAMV - Iaşi Seria Agronomie 59(1): 73-76.
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1464
dc.description.abstract The aim of the study was to investigate serological and molecula detection of Cucumber mosaic virus (CMV) in pepper growing areas in Isparta province, Turkey. During surveys conducted in 2013- 2014 growing season, field-grown pepper plants with symptoms of mosaic, chlorotic mottling, vein banding, blistering and leaf and fruit deformation were observed. A total of 239 leaf samples were collected from various locations in this region. The Leaf samples were tested by DAS-ELISA (Double antibody Sandwich Enzyme Linked Immunosorbent Assay) using specific kits to detect CMV. ELISA tests showed that among 239 samples, 10 were infected with CMV. To confirm the results of ELISA positive leaf samples were tested by RT-PCR method and the respected size of band with 678 bp was observed on agarose gel electrophoresis for CMV. As a result of the studies, ELISA test results and RT-PCR test results were confirmed each other. en_US
dc.language.iso en en_US
dc.publisher “Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iaşi en_US
dc.subject pepper en_US
dc.subject Cucumber mosaic virus en_US
dc.subject ELISA en_US
dc.subject RT-PCR en_US
dc.title Molecular and serological detection of cucumber mosaic virus in pepper growing areas in Isparta province, Turkey en_US
dc.type Article en_US
dc.author.affiliation Handan Çulal-Kiliç, Nejla Yardimci, Süleyman Demirel University, Agricultural Faculty, Plant Protection Department, Isparta-Turkey
dc.publicationName Lucrări Ştiinţifice USAMV - Iaşi Seria Agronomie
dc.volume 59
dc.issue 1
dc.publicationDate 2016
dc.startingPage 73
dc.endingPage 76
dc.identifier.eissn 2069-6727


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