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Separation and characterisation of the main proanthocyanidin fractions of grape seeds

Show simple item record Filimon, V. R. Nechita, Ancuta Damian, Doina Pașa, Rodica Filimon, Roxana Băetu, M. Mihai, C. T. Niculaua, M. 2021-05-14T10:13:46Z 2021-05-14T10:13:46Z 2017
dc.identifier.citation Filimon V. R., Ancuța Nechita, Doina Damian, Rodica Pașa, Roxana Filimon, M. Băetu, C. T. Mihai, M. Niculaua. 2017. "Separation and characterisation of the main proanthocyanidin fractions of grape seeds". Lucrări Știinţifice USAMV – Iași Seria Horticultură, 60(1): 69-74.
dc.description.abstract Grape seeds, as waste products of the winemaking industry, contain large amounts of monomers, oligomers and more highly polymerised proanthocyanidins (PA), being a good source of phytochemicals for the production of antioxidative dietary supplements. PA from defatted grape seeds were extracted by precipitation with diethyl ether from the crude alcoholic extract and fractionated into monomers (FI), oligomers (FII) and polymers (FIII) of flavan-3-ols by their separation on C18 Sep-Pak cartridges. FIII was the predominant class of proanthocyanidins (82.22%), while monomeric PA has only 5.71% of total. The ratio PA (by vanillin assay) / tannins (Bate-Smith assay) indicates the highest degree of polymerisation (DP) in FIII fraction (1.28). Thin layer chromatography (TLC) confirmed the presence of monomers in FI, the DP increasing significantly for the next two fractions. Oligomeric and polymeric PA showed the highest antioxidant activity (% scavenged DPPH), but the synergic antioxidant effect of PA classes was also observed. en_US
dc.language.iso en en_US
dc.publisher “Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași en_US
dc.subject antioxidant activity en_US
dc.subject C18 cartridges en_US
dc.subject degree of polymerisation en_US
dc.subject grape seeds en_US
dc.subject tannins en_US
dc.title Separation and characterisation of the main proanthocyanidin fractions of grape seeds en_US
dc.title.alternative Separarea şi caracterizarea principalelor fracţiuni de proantocianidine din seminţele de struguri en_US
dc.type Article en_US V. R. Filimon, A. Nechita, D. Damian, R. Pașa, R. Filimon, M. Băetu, Research-Development Station for Viticulture and Winemaking Iasi, Romania C. T. Mihai, Biological Research Institute Iasi, Romania M. Niculaua, Research Center for Oenology of Romanian Academy -Iasi Branch, Romania
dc.publicationName Lucrări Știinţifice USAMV – Iași Seria Horticultură
dc.volume 60
dc.issue 1
dc.publicationDate 2017
dc.startingPage 69
dc.endingPage 74
dc.identifier.eissn 2069-8275

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