ALSERepository of Iași University of Life Sciences, ROMANIA

Observations regarding in vitro hatching of Raillietina spp. (Cestoda: Cyclophyllidea) onchosphere

Show simple item record

dc.contributor.author Roman, Constantin
dc.contributor.author Ciucă, Lavinia
dc.contributor.author Mardare, Raluca
dc.contributor.author Lupu, Andrei
dc.contributor.author Cîmpan, Andrei
dc.contributor.author Ivănescu, Larisa
dc.contributor.author Iacob, Olimpia
dc.contributor.author Acatrinei, Dumitru
dc.contributor.author Miron, Liviu
dc.date.accessioned 2021-05-12T10:02:40Z
dc.date.available 2021-05-12T10:02:40Z
dc.date.issued 2017
dc.identifier.citation Roman, Constantin, Lavinia Ciucă, Raluca Mardare, Andrei Lupu, Andrei Cîmpan, Larisa Ivănescu, Olimpia Iacob, Dumitru Acatrinei, Liviu Miron. 2017. "Observations regarding in vitro hatching of Raillietina spp. (Cestoda: Cyclophyllidea) onchosphere". Lucrări Științifice USAMV - Iași Seria Medicină Veterinară 60 (4): 477-480. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1233
dc.description.abstract The study has been realised in May 2016 having as purpose the observation of the onchosphere hatching process. The eggs of Raillietina spp. were aquired from feces belonging to Great Spotted Woodpecker (Dendrocopos major) that was naturaly infested. Initally, the samples were examined from a parasitological point of view using the Willis method. The egg concentrate has been obtained using the protocol described by VOGE and all. (1961) with some adjustments. The egg suspension was incubated at 30 oC for an hour. The hatching process of the oncosphere has been observed on the slide with the optical microscope (Motic B series) fitted with a Moticam 1000 camera, using the x400 magnification. Measuring has been realised with Motic Images Plus 2.0 software. The temperature of the slide in the moment of examination was 32oC. The whole hatching proces lasted 5 hours and went through the following stages: after half an hour the hexachant embryo has broken the basal membrane and entered the vitelline layer, the onchosphere was vigorously pushing and scraping the granular structure belonging to the vitelline layer, then the vitelline membrane and the egg shell was perforated after four and a half hours, thus completing the actual hatching process. After hatching, the onchosphere engaged in a series of swim-like motions in the liquid mass. en_US
dc.language.iso en en_US
dc.publisher ”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iasi en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Raillietina spp. en_US
dc.subject hatching oncosphere en_US
dc.subject great spotted woodpecker en_US
dc.title Observations regarding in vitro hatching of Raillietina spp. (Cestoda: Cyclophyllidea) onchosphere en_US
dc.type Article en_US
dc.author.affiliation Constantin Roman, Lavinia Ciucă, Raluca Mardare, Andrei Lupu, Andrei Cîmpan, Larisa Ivănescu, Olimpia Iacob, Dumitru Acatrinei, Liviu Miron, University of Agricultural Sciences and Veterinary Medicine “Ion Ionescu de la Brad”, Faculty of Veterinary Medicine, Iași, România
dc.publicationName Lucrări Științifice USAMV - Iași, Seria Medicină Veterinară
dc.volume 60
dc.issue 4
dc.publicationDate 2017
dc.startingPage 477
dc.endingPage 480
dc.identifier.eissn 2393-4603


Files in this item

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)