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Cell growth characteristics of equine synovial fluid stem cells

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dc.contributor.author Pall, Emoke
dc.contributor.author Pall, Klementina Katalin
dc.contributor.author Crecan, Cristian
dc.contributor.author Ciupe, Simona
dc.contributor.author Cenariu, Mihai
dc.contributor.author Groza, Ioan-Ștefan
dc.date.accessioned 2021-05-11T11:55:59Z
dc.date.available 2021-05-11T11:55:59Z
dc.date.issued 2017
dc.identifier.citation Pall, Emoke, Klementina Katalin Pall, Cristian Crecan, Simona Ciupe, Mihai Cenariu, Ioan Groza. 2017. "Cell growth characteristics of equine synovial fluid stem cells". Lucrări Științifice USAMV - Iași Seria Medicină Veterinară 60 (3): 369-373. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1210
dc.description.abstract Equine mesenchymal stem cells (MSCs) have been isolated from various sources such as: peripheral blood, bone marrow, adipose tissue, umbilical cord, placenta, Wharton Jelly. Besides these synovial fluid and synovial membrane represents a promising source of mesenchymal stem cells, which can be harvested by minimally invasive methods. MSCs from these sources have the potential to self-renew and differentiate into multiple lineages such as chondrogenic, adipogenic and osteogenic. The aim of our study was to evaluate the growth characteristics of equine synovial liquid stem cells harvested from the tarsometatarsal joint during arthroscopic surgery. Samples were collected in a sterile syringe and were diluted and centrifuged at 1500rpm for 7 min. The supernatant were removed and the cells were resuspended in propagation medium: Dulbecco’s Modified Eagle’s Medium/F12 (DMEM/F12, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% antibiotic-antimycotic (Sigma-Aldrich). The medium was changed after 3 days. The proliferation ability, cell doubling number, cell doubling time, daily duplication rate and clonogenic efficacy was evaluated. Isolated cells exhibited plastic adherence capacity, monolayer growth, and fibroblast-like morphology, high growth and clonogenic capacity. Our study demonstrated the characteristics of equine synovial fluid derived stem cells, an ideal candidate for veterinary regenerative medicine. en_US
dc.language.iso en en_US
dc.publisher ”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iasi en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject stem cells en_US
dc.subject synovial fluid en_US
dc.subject horse en_US
dc.subject proliferation en_US
dc.subject cells growth en_US
dc.title Cell growth characteristics of equine synovial fluid stem cells en_US
dc.type Article en_US
dc.author.affiliation Emoke Pall, Cristian Crecan, Simona Ciupe, Mihai Cenariu, Ioan Groza, University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Cluj-Napoca, Mănăştur, no. 3-5, 400372, Cluj-Napoca, Romania
dc.author.affiliation Emoke Pall, Klementina Katalin Pall, University of Szent Istvan, Godollo, Hungary
dc.publicationName Lucrări Științifice USAMV - Iași, Seria Medicină Veterinară
dc.volume 60
dc.issue 3
dc.publicationDate 2017
dc.startingPage 369
dc.endingPage 373
dc.identifier.eissn 2393-4603


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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)