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Evaluation of sows oocytes viability through Trypan Blue staining after vitrification

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dc.contributor.author Marc, Simona
dc.contributor.author Mircu, C.
dc.contributor.author Crețan, Nicoleta
dc.contributor.author Otavă, G.
dc.contributor.author Tulcan, Camelia
dc.contributor.author Huțu, I.
dc.date.accessioned 2021-05-11T11:39:54Z
dc.date.available 2021-05-11T11:39:54Z
dc.date.issued 2017
dc.identifier.citation Marc, Simona, C. Mircu, Nicoleta Crețan, G. Otavă, Camelia Tulcan, I. Huțu. 2017. "Evaluation of sows oocytes viability through Trypan Blue staining after vitrification". Lucrări Științifice USAMV - Iași Seria Medicină Veterinară 60 (3): 359-364. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1208
dc.description.abstract Along sperm and embryo cryopreservation that are used routinely also in animal assisted reproduction, studying are done also on animal oocyte cryopreservation in order to find the best conditions to preserve their viability. Vitrification is one of the methods that can be used in order to preserve oocytes. The higher reactive oxygen species that are formed during in vitro conditions can influence the success of assisted reproduction technique. The aim of this study was to evaluate the antioxidant potential of ascorbic acid (0.5mM) and rosmarinic acid (105μM) added in media for in vitro maturation on swine oocyte subjected to vitrification. COC’s viability after vitrification was evaluated by 0.02% Trypan Blue staining. Comparing experimental groups C (vitamin C) and AR (rosmarinic acid) with group M (control), relative to the number of vitrified oocytes, a slight increase in their viability is observed, with 16.67% (C, class I) and 33.33% (AR, class II), respectively. Regardless of the treatment applied, the oocyte class is associated with viability (p = 0.048). Due to low number of oocytes used in each group we can concluded that supplementation of oocyte maturation media before vitrification with rosmarinic acid and ascorbic acid could produce a slight increase in viability. en_US
dc.language.iso en en_US
dc.publisher ”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iasi en_US
dc.subject antioxidants en_US
dc.subject oocyte en_US
dc.subject vitrification en_US
dc.title Evaluation of sows oocytes viability through Trypan Blue staining after vitrification en_US
dc.type Article en_US
dc.author.affiliation Simona Marc, C. Mircu, Nicoleta Crețan, G. Otavă, Camelia Tulcan, I. Huțu, University of Agricultural Sciences and Veterinary Medicine of Banat “The King of Michael I” of Romania, Faculty of Veterinary Medicine, Timisoara, Romania
dc.publicationName Lucrări Științifice USAMV - Iași, Seria Medicină Veterinară
dc.volume 60
dc.issue 3
dc.publicationDate 2017
dc.startingPage 359
dc.endingPage 364
dc.identifier.eissn 2393-4603


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