ALSERepository of Iași University of Life Sciences, ROMANIA

Study of the anti-pregnancy - associated glycoprotein (anti-PAG) resulted from antigen PAG immunization, as prospective early pregnancy detector in animals

Show simple item record

dc.contributor.author Lestari, Tita Damayanti
dc.date.accessioned 2021-05-10T11:05:27Z
dc.date.available 2021-05-10T11:05:27Z
dc.date.issued 2016-10-31
dc.identifier.citation Lestari, Tita Damayanti. 2016. ”Study of the anti-pregnancy - associated glycoprotein (anti-PAG) resulted from antigen PAG immunization, as prospective early pregnancy detector in animals”. Cercetări Agronomice în Moldova 49 (3): 111-118. DOI: 10.1515/cerce-2016-0030. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1189
dc.description.abstract Blastocyst protein named pregnancy-associated glycoprotein (PAG) has been isolated from pregnant dairy cow serum. PAG yielded from previous research issued from pregnant animals, wherein was specifically produced. This study is an initial research to use PAG as an antigen to be injected to rabbit in order to produce the corresponding antibody. The objective of the research was to study the character of anti-pregnancy-associated glycoprotein (Anti-PAG), as a result of immunization of PAG and to learn its specification reaction. The PAG isolate had bovine origin (molecular weight-MW of 67.34 kDa) and issued from previous research. Injection of PAG isolate could stimulate the production of Anti-PAG as an immunization response. Immunization was done by double booster inoculation. Anti-PAG derived from immunization was characterized via antibody titer value using ELISA technique. Specificity test of Anti-PAG was carried on by Western Blot technique. Results revealed that injection of PAG isolate to the rabbit stimulates anti-PAG production. Concentration of Anti-PAG was 1.192 μg with titer of 1.044. Anti-PAG derived from rabbits recognized Standard PAG antigen (bovine PAG MDBiomed cat. 101-7963-13- 3) and PAG isolate derived from previous research. The reaction of antigen antibody is the basic structure of creating a gestation detection kit. Anti-PAG is expected to be the molecular marker in developing a gestation detection kit in the near future. en_US
dc.language.iso en en_US
dc.publisher "Ion Ionescu de la Brad" University of Agricultural Sciences and Veterinary Medicine, Iași en_US
dc.subject protein pregnancy-associated glycoprotein (PAG) en_US
dc.subject cow serum en_US
dc.subject immunization en_US
dc.subject rabbit en_US
dc.subject Anti-PAG en_US
dc.title Study of the anti-pregnancy - associated glycoprotein (anti-PAG) resulted from antigen PAG immunization, as prospective early pregnancy detector in animals en_US
dc.type Article en_US
dc.author.affiliation Tita Damayanti Lestari, Department of Veterinary Reproduction, Faculty of Veterinary Medicine Universitas Airlangga, Surabaya, East Java, Indonesia
dc.publicationName Cercetări Agronomice în Moldova
dc.volume 49
dc.issue 3
dc.publicationDate 2016
dc.startingPage 111
dc.endingPage 118
dc.identifier.eissn 2067-1865
dc.identifier.doi 10.1515/cerce-2016-0030


Files in this item

This item appears in the following Collection(s)

Show simple item record