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INA gene inactivation in isolated strains from frozen leaves and its effects on plant freezing

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dc.contributor.author Yadollahpour, A.
dc.contributor.author Bagheri, N.A.
dc.contributor.author Rahimian, H.
dc.date.accessioned 2021-05-10T09:01:46Z
dc.date.available 2021-05-10T09:01:46Z
dc.date.issued 2016-10-31
dc.identifier.citation Yadollahpour, A., N.A. Bagheri, H. Rahimian. 2016. ”INA gene inactivation in isolated strains from frozen leaves and its effects on plant freezing”. Cercetări Agronomice în Moldova 49 (3): 63-70. DOI: 10.1515/cerce-2016-0026. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1178
dc.description.abstract Freezing is a major environmental stress, which limits plant’s distribution, growth and productivity. Ice nucleation active bacteria can catalyze ice formation at temperatures as high as -2°C. A membrane protein confer the ability of ice nucleation, called ice-nucleating proteins (INPs), which is encoded by a single gene. Mutation in this gene will lead to delaying of ice nucleation. In this study, leaf tissues of several plants with freezing symptoms were collected from different locations and 40 bacterial isolates with yellow circular colonies and regular margins were isolated from samples. Finally, total of 12 isolates belong to Xanthomonas were selected for ice nucleate activity (INA) by Dropletfreezing test and presence of INA gene was surveyed by PCR. According to the obtained results, isolate 28 was targeted to mutagenesis by using Tn5 transposon. After mutagenesis, isolates with ability to grow on kanamycin, which lack of INAx gene in PCR were considered as mutated isolates and their freezing effects were evaluated on bean seedlings. Results showed that isolates with mutated INA gene cannot induce freezing on bean seedlings, while primary identified isolate (isolate 28) could do it. These results show that if we could replace wild type ice nucleation active bacteria with mutated forms (just different in ice nucleation activity), we could, probably, prevent freezing and subsequent economic losses. en_US
dc.language.iso en en_US
dc.publisher "Ion Ionescu de la Brad" University of Agricultural Sciences and Veterinary Medicine, Iași en_US
dc.subject freezing stress en_US
dc.subject ice nucleating protein en_US
dc.subject mutagenesis en_US
dc.subject transposon en_US
dc.subject Xanthomonas en_US
dc.title INA gene inactivation in isolated strains from frozen leaves and its effects on plant freezing en_US
dc.type Article en_US
dc.author.affiliation A. Yadollahpour, N.A. Bagheri, Agronomy and Plant Breeding Department, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
dc.author.affiliation H. Rahimian, Plant Protection Department, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
dc.publicationName Cercetări Agronomice în Moldova
dc.volume 49
dc.issue 3
dc.publicationDate 2016
dc.startingPage 63
dc.endingPage 70
dc.identifier.eissn 2067-1865
dc.identifier.doi 10.1515/cerce-2016-0026


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