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Optimization of sweet potato (Ipomoea batatas L.) in vitro cultivation by using the contaminated cultures for obtaining new shoots in greenhouse conditions

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dc.contributor.author Cioloca, Mihaela
dc.contributor.author Tican, Andreea
dc.contributor.author Popa, Monica
dc.contributor.author Bărăscu, Nina
dc.date.accessioned 2021-05-07T05:53:10Z
dc.date.available 2021-05-07T05:53:10Z
dc.date.issued 2020
dc.identifier.citation Cioloca, Mihaela, Andreea Tican, Monica Popa, Nina Bărăscu. 2020. "Optimization of sweet potato (Ipomoea batatas L.) in vitro cultivation by using the contaminated cultures for obtaining new shoots in greenhouse conditions". Lucrări Ştiinţifice USAMV - Iaşi Seria Agronomie 63(2): 125-130.
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1118
dc.description.abstract The artificial media used for plant tissue cultures contains numerous nutrients that can create favorable conditions for the development of pathogens. As antibiotic use is not encouraged, a new strategy has been tried to reduce the losses caused by the presence of microbial infections in "in vitro" cultivation of sweet potato. This consisted in the transplantation in greenhouse conditions of sweet potato plantlets from contaminated "in vitro" cultures, in order to obtain new shoots. Five sweet potato varieties were used in this study, and two types of substrate for planting: pearlite and a mixture of peat and pearlite (1:1). The survival rate of the plantlets was 100% on the substrate consisting only of pearlite. The surviving sweet potato plants had a rapid growth rate, the greenhouse conditions being favorable for this culture. Approximately two months after transplantation, the obtained shoots could be used as a source of explants to initiate new "in vitro" cultures. Regarding the number of shoots the highest value was obtained by the Yulmi variety. The length of the shoots varied according to the variety, thus the highest value was recorded by the KSC1 variety (98.30 cm) . The number of buds/shoot is strongly influenced by the variety. In some sweet potato varieties the distance between buds is smaller, and in others larger, this being a characteristic of the variety. Regarding this trait the best results were obtained in Juhwangmi variety. By applying this method, the process of sweet potato "in vitro" multiplication becomes more economically efficient. After only a few weeks under greenhouse conditions, involving minimal costs, many shoots can be obtained. en_US
dc.language.iso en en_US
dc.publisher “Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iaşi en_US
dc.subject plant tissue culture en_US
dc.subject microbial contamination en_US
dc.subject plantlets en_US
dc.subject greenhouse en_US
dc.subject sweet potato en_US
dc.title Optimization of sweet potato (Ipomoea batatas L.) in vitro cultivation by using the contaminated cultures for obtaining new shoots in greenhouse conditions en_US
dc.type Article en_US
dc.author.affiliation Mihaela Cioloca, Andreea Tican, Monica Popa, Nina Bărăscu, National Institute for Research and Development for Potato and Sugar Beet, Brasov
dc.publicationName Lucrări Ştiinţifice USAMV - Iaşi Seria Agronomie
dc.volume 63
dc.issue 2
dc.publicationDate 2020
dc.startingPage 125
dc.endingPage 130
dc.identifier.eissn 2069-6727


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