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Molecular studies on Pasteurella species isolated from ducks

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dc.contributor.author Amany, O.S.
dc.contributor.author Alrafie, Amira S.
dc.contributor.author Sabry, E.O.
dc.contributor.author Elsayed, Hemat Sh.
dc.date.accessioned 2021-05-07T05:45:53Z
dc.date.available 2021-05-07T05:45:53Z
dc.date.issued 2017
dc.identifier.citation Amany, O.S., Amira S. Alrafie, E.O. Sabry, Hemat Sh. Elsayed. 2017. "Molecular studies on Pasteurella species isolated from ducks". Lucrări Științifice USAMV - Iași Seria Medicină Veterinară 60 (1): 56-64. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1117
dc.description.abstract Duck cholera is a fatal, contagious and septicemic disease of ducks caused by Pasteurella species. A total of 150 ducks were collected from ten farms in Kaliobia Governorate suspected to be suffering from Pasteurellosis that manifested by respiratory signs, sudden death, and nervous manifestation. Collected Samples from these ducks were liver, spleen, heart and lung which subjected for bacteriological examination. A total of 33 Pasteurella strains were isolated, 25 strain were Pasteurella multocida (recovered from liver samples) and 8 strain were Pasteurella pneumotropica (5 strains recovered from lung and 3 strain recovered from heart). Finding of antibiotic sensitivity test showed that Pastreulla isolates were sensitive to florofinicole (80%) and moderately sensitive to ciprofloxacine (60%), enrofloxacin (50%) and followed by tobramycin (40%). Amoxicillin, oxytetracycline and penicillin were less sensitive (30% each) while isolates showed absolute resistance to erythromycin (100%) followed by resistance to gentamycin (90%) and naldixic acid (80%) for both types of Pasteurella. PCR results showed that Cytotoxic protein (toxA) toxcin virulence gene was detected in 4 out of 10 studied strains and fimbrial protein (ptfA) virulence gene was detected in 4 out of 10 studied strains. Sequences of toxA and ptfA genes were submitted to Gen Bank and assigned accession numbers were MF167359 and MF382009, respectively. en_US
dc.language.iso en en_US
dc.publisher ”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Pasteurella multocida en_US
dc.subject Pasteurella pneumotropica en_US
dc.subject toxA en_US
dc.subject ptfA en_US
dc.subject antibiotic sensitivity test en_US
dc.subject PCR en_US
dc.subject ducks en_US
dc.title Molecular studies on Pasteurella species isolated from ducks en_US
dc.type Article en_US
dc.author.affiliation Amira S. Alrafie, E.O. Sabry, Poultry diseases Department
dc.publicationName Lucrări Științifice USAMV - Iași, Seria Medicină Veterinară
dc.volume 60
dc.issue 1
dc.publicationDate 2017
dc.startingPage 56
dc.endingPage 64
dc.identifier.eissn 2393-4603
alse.contributor.author O.S. Amany, E.O. Sabry, Hemat Sh. Elsayed, Animal Health Research Institute Banha
alse.contributor.author Amira S. Alrafie, Zagazig Branch, Egypt
alse.contributor.author O.S. Amany, Amira S. Alrafie, Microbiology Department


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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)