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Preliminary studies regarding antimicrobial effect of various kuwanon G – antibiotic combinations on some MRSA strains

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dc.contributor.author Horhogea, Cristina
dc.contributor.author Rîmbu, Cristina
dc.contributor.author Aelenei, Petruța
dc.contributor.author Guguianu, Eleonora
dc.contributor.author Crețu, Carmen
dc.contributor.author Dimitriu, Gabriel
dc.contributor.author Miron, Anca
dc.date.accessioned 2021-05-06T06:51:15Z
dc.date.available 2021-05-06T06:51:15Z
dc.date.issued 2017
dc.identifier.citation . Horhogea, Cristina, Cristina Rîmbu, Petruța Aelenei, Eleonora Guguianu, Carmen Crețu, Gabriel Dimitriu, Anca Miron. 2017. "Preliminary studies regarding antimicrobial effect of various kuwanon G – antibiotic combinations on some MRSA strains". Lucrări Științifice USAMV - Iași Seria Medicină Veterinară 60 (1): 30-38. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1088
dc.description.abstract Methicillin-resistant Staphylococcus aureus (MRSA) is a constant therapeutic challenge in humans and animals, due to the limited range of antibiotics that can be used for the management of infections. This preliminary study is based on the assessment of the antibacterial activity of kuwanon G (a prenylated flavonoid present in white mulberry, Morus alba L., Moraceae) and its interactions with various antibiotics (oxacillin, amoxicillin, erythromycin and gentamicin) against four MRSA clinical isolates (MRSA T1 – T4). The sources of all clinical isolates resistant to cefoxitin and oxacillin were infections (recurrent otitis, pyoderma and laryngopharyngitis) in dogs. Minimum inhibitory concentrations (MICs) for kuwanon G and antibiotics were determined by the microdilution method. Interactions between kuwanon G and antibiotics were evaluated by the checkerboard method and time-kill assay. MICs varied between 6.25 and 12.5μg/mL for kuwanon G alone against all four MRSA clinical isolates. According to the calculated fractional inhibitory concentration index, various combinations were synergistic and additive. Microbicidial time has confirmed the synergy as the logarithmic reductions of colony-forming units obtained for the combinations of kuwanon G and some antibiotics were 2log10 lower than the logarithmic reductions obtained for the most potent/active component of the combination. The obtained results are promising, taking into account the antibacterial activity of kuwanon G, as well as its synergistic effects with the most used antibiotics. This study reports on the antibacterial activity of kuwanon G and suggests its ability to act synergistically with antibiotics; combinations effective in combating Gram-positive including MRSA infections might be developed. en_US
dc.language.iso en en_US
dc.publisher ”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject checkerboard en_US
dc.subject kuwanon G en_US
dc.subject MRSA en_US
dc.subject synergy en_US
dc.subject time-kill assay en_US
dc.title Preliminary studies regarding antimicrobial effect of various kuwanon G – antibiotic combinations on some MRSA strains en_US
dc.type Article en_US
dc.author.affiliation Cristina Horhogea, Cristina Rîmbu, Eleonora Guguianu, Carmen Crețu, Microbiology-Immunology Laboratory, Department of Public Health, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Ion Ionescu de la Brad, Iasi, Romania
dc.author.affiliation Petruța Aelenei, Anca Miron, Department of Pharmacognosy, Faculty of Pharmacy, University of Medicine and Pharmacy Grigore T. Popa, Iasi, Romania
dc.author.affiliation Gabriel Dimitriu, Department of Preventive Medicine and Interdisciplinary, Discipline Medical Informatics and Biostatistics, Faculty of Medicine, University of Medicine and Pharmacy Grigore T. Popa, Iasi, Romania
dc.publicationName Lucrări Științifice USAMV - Iași, Seria Medicină Veterinară
dc.volume 60
dc.issue 1
dc.publicationDate 2017
dc.startingPage 30
dc.endingPage 38
dc.identifier.eissn 2393-4603


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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)