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Effective diagnostic techniques in Borrelia burgdorferi infestation in dogs

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dc.contributor.author Ivănescu, Maria-Larisa
dc.contributor.author Martinescu, Gabriela-Victoria
dc.contributor.author Miron, Liviu-Dan
dc.date.accessioned 2023-07-12T10:58:13Z
dc.date.available 2023-07-12T10:58:13Z
dc.date.issued 2023-02-09
dc.identifier.citation Ivănescu, Maria Larisa, Gabriela Martinescu, Liviu Dan Miron. 2022. “Effective diagnostic techniques in Borrelia burgdorferi infestation in dogs”. Journal of Applied Life Sciences and Environment 55 (2): 219-232. https://doi.org/10.46909/alse-552059. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/3353
dc.description.abstract Borreliosis or Lyme disease is a disease transmitted by ixodidae ticks during feeding on blood (Ixodes pacificus and Ixodes scapularis in the USA, Ixodes persulcatus in Asia, Ixodes ricinus in Europe) and is widespread in the entire northern hemisphere. In Romania, the geographic distribution and prevalence of Borrelia burgdorferi sensu lato was 1.4% in 41 counties, with a prevalence between 0.75–18.8%. B. burgdorferi sensu lato. had a prevalence of 3.8%, being found inside ticks in 55 of 183 localities. Successful treatment and full recovery can only be achieved through early diagnosis. The clinical and serologic diagnosis of Lyme disease is particularly difficult because of the phenotypic heterogeneity within and among spirochete species. A case study is presented in this paper: an eight-year-old male Yorkshire terrier dog, which was diagnosed positive for Lyme disease, based on a test which uses a peptide called C6 and which comes from the VlsE protein of B. burgdorferi, used to detect antibodies in dogs. The results demonstrate the reliability of the commercial SNAP 4Dx Plus Test for B. burgdorferi, which uses C6 to differentiate antibodies produced by natural infection from antibodies formed after vaccination. In addition, using real-time PCR, the diagnosis was negative, confirming the results from the literature, according to which the PCR technique is only recommended for research, the positivity percentage being low, especially when the sample is blood (0.1%). We conclude that the tests for the detection of antibodies specific to Lyme disease are recommended and useful. en_US
dc.language.iso en en_US
dc.publisher Iasi University of Life Sciences (IULS), Iași en_US
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri http://creativecommons.org/licenses/by/4.0
dc.subject Lyme disease en_US
dc.subject antibodies en_US
dc.subject real-time PCR en_US
dc.title Effective diagnostic techniques in Borrelia burgdorferi infestation in dogs en_US
dc.type Article en_US
dc.author.affiliation Maria Larisa Ivănescu, Gabriela Martinescu, Liviu Dan Miron, Iasi University of Life Sciences, Faculty of Veterinary Medicine, Department of Clinics, 8, Mihail Sadoveanu Alley, 700489, Iasi, Romania
dc.publicationName Journal of Applied Life Sciences and Environment
dc.volume 55
dc.issue 2
dc.publicationDate 2022
dc.startingPage 219
dc.endingPage 232
dc.identifier.eissn 2784 - 0360
dc.identifier.doi 10.46909/alse-552059


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Attribution 4.0 International (CC BY 4.0) Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)