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Salinity effect on the growth of rhizobia in vitro

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dc.contributor.author Niste, Monica
dc.contributor.author Vidican, Roxana
dc.contributor.author Rotar, Ioan
dc.contributor.author Miclea, Raluca
dc.contributor.author Pop, Rodica
dc.date.accessioned 2021-10-19T10:14:07Z
dc.date.available 2021-10-19T10:14:07Z
dc.date.issued 2015
dc.identifier.citation Niste, Monica, Roxana Vidican, Ioan Rotar, Raluca Miclea, Rodica Pop. 2015. "Salinity effect on the growth of rhizobia in vitro". Lucrări Ştiinţifice USAMV - Iaşi Seria Agronomie 58(1): 63-65.
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1943
dc.description.abstract This study aimed to isolate and collect different strains from soil and to characterize their performance against salinity. Two strains Rhizobium leguminosarum bv. trifolii and two Sinorhizobium meliloti strains were used in this study to characterize the growth performance against salinity. Nodules collected from this plants were surface sterilized and presumptive test for rhizobia was conducted before authentication according to Somasegran and Hoben (1994). Yeast extract-mannitol medium (YEM), was used for routine cultivation of rhizobia, and when necessary the medium was solidified with 1.2 % agar (YEMA). To test the rhizobial growth at different salt concentration we supplemented the medium by adding 0, 50, 100, 150 and 300 mM sodium chloride (NaCl). Rhizobial growth after incubation at 28°C with shaking for three days was measured spectrofotometrically at OD600 nm. Rhizobial strains were classified by their growth response at 300 mM regarding their optical density (OD). Result showed the two Rhizobium leguminosarum bv. trifolii strains tested , about half were able to tolerate up to 300 mM of salt, and the best performance strains were RtR 2. Tolerance of high levels of NaCl varies for Sinorhizobium meliloti and all strains showed good salt tolerance efficiency compared with Rhizobium trifolii. The best Sinorhizobium meliloti strain was SmM 2. The two rhizobia species examined best development has been observed on the Sinorhizobium meliloti. At the other species studied in the presence of salt was observed that the number of colony development was adversely affected. These salt tolerant strains are excellent models to study the mechanisms of the resistance and the impact of NaCl on rhizobia tolerance. en_US
dc.language.iso en en_US
dc.publisher “Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iaşi en_US
dc.subject Rhizobium trifolii en_US
dc.subject Sinorhizobium meliloti en_US
dc.subject sodium chloride en_US
dc.subject tolerance en_US
dc.title Salinity effect on the growth of rhizobia in vitro en_US
dc.type Article en_US
dc.author.affiliation Monica Niste, Roxana Vidican, Ioan Rotar, Raluca Miclea, Rodica Pop, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca
dc.publicationName Lucrări Ştiinţifice USAMV - Iaşi Seria Agronomie
dc.volume 58
dc.issue 1
dc.publicationDate 2015
dc.startingPage 63
dc.endingPage 68
dc.identifier.eissn 2069-6727


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