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Quality evaluation of freeze-dried ammodytes ammodytes venom by CGE on-a chip with laser induced fluorescence detection

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dc.contributor.author Tulcan, Camelia
dc.contributor.author Tudor, George
dc.contributor.author Boldura, Oana-Maria
dc.contributor.author Ahmadi, Mirela
dc.contributor.author Mircu, Călin
dc.contributor.author Huțu, Ioan
dc.contributor.author Milovanov, Cornelia
dc.contributor.author Sărăndan, Mihai
dc.contributor.author Marc Zarcula, Simona
dc.date.accessioned 2021-10-06T07:13:08Z
dc.date.available 2021-10-06T07:13:08Z
dc.date.issued 2015
dc.identifier.citation Tulcan, Camelia, George Tudor, Oana Boldura, Mirela Ahmadi, Calin Mircu, Ioan Hutu, Cornelia Milovanov, Mihai Sărăndan, Simona Zarcula. 2015. "Quality evaluation of freeze-dried ammodytes ammodytes venom by CGE on-a chip with laser induced fluorescence detection". Lucrări Științifice USAMV-Iași Seria Medicină Veterinară 58 (1): 120-126. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1886
dc.description.abstract Our study represents the first attempt to develop a protocol for evaluating the quality freeze- dried venom by using current proteomics analysis techniques The research carried out represents the initial step in the validation of the method for protein analysis by capillary electrophoresis gel with laser induced fluorescence detection , being our first attempt in determine the impairment of protein fractions during long storage of the freeze-dried venom . Freeze-drying was used as a method of advanced dehydration Ammodytes ammodytes venom and the yield of these procedure went up to 25.92 %. On a long-term storage at -20 ° C freeze-dried venom of the factions protein degradation occurs , the most affected being that with the molecular weight of approx . 28 and 37 kDa , respectively identified as Vammin and phospholipase A2 , as demonstrated on the one hand, by decreasing the % of these fractions and on the other hand, by increasing the % of protein fractions obtained by degradation. en_US
dc.language.iso en en_US
dc.publisher ”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject ammodytes ammodytes venom en_US
dc.subject freeze-dry en_US
dc.subject CGE-on a chip en_US
dc.title Quality evaluation of freeze-dried ammodytes ammodytes venom by CGE on-a chip with laser induced fluorescence detection en_US
dc.type Article en_US
dc.author.affiliation Camelia Tulcan, George Tudor, Oana Boldura, Mirela Ahmadi, Calin Mircu, Ioan Hutu, Cornelia Milovanov, Mihai Sărăndan, Simona Zarcula, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine of Banat “The King of Michael I of Romania” from Timișoara, Romania
dc.publicationName Lucrări Științifice USAMV - Iași, Seria Medicină Veterinară
dc.volume 58
dc.issue 1
dc.publicationDate 2015
dc.startingPage 120
dc.endingPage 126
dc.identifier.eissn 2393-4603


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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)