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Nanoscale level determination methods of endocrine disruptors in animal fluids, and their effect upon the female genital tract

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dc.contributor.author Ilaș, Vlad-Alexandru
dc.contributor.author Henegariu, Adrian
dc.contributor.author Cenariu, Mihai
dc.contributor.author Pall, Emoke
dc.contributor.author Groza, Ioan-Ștefan
dc.date.accessioned 2021-07-09T11:20:43Z
dc.date.available 2021-07-09T11:20:43Z
dc.date.issued 2016
dc.identifier.citation Ilaș, Vlad, Adrian Henegariu, Mihai Cenariu, Emoke Pall, Ioan Groza. 2016. "Nanoscale level determination methods of endocrine disruptors in animal fluids, and their effect upon the female genital tract". Lucrări Științifice USAMV-Iași Seria Medicină Veterinară 59 (4): 458-462. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1692
dc.description.abstract compounds. A wide variety of environmental contaminants disrupt endocrine system of many species (cattle, sheep, horses) including human, known as endocrine disruptors. Organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs), grouped collectively as organochlorine compounds (OCCs) and heavy metals (HMs), are the most widespread and persistent, being lipophilic and slowly biodegradable. Being ubiquitous in the environment, these compounds are implicitly present in the feed. The main goal of this paper was to determine nanoscale levels of endocrine disruptors in animal fluids (serum/plasma) and risk assessment on the female reproductive system. The blood samples were collected from cows belonging to a private farmer near Cluj-Napoca. Different aged cows were selected, from which we collected 25 ml of blood in tubes containing an anti-clotting agent and 10 ml in tubes with no additives. Extracted plasma and serum were analysed using the following methods: Method for determination of heavy metals in serum/plasma by microwave digestion and inductively coupled plasma atomic emission spectrometry (MW-ICP-AES); Method for simultaneous determination of polychlorinated biphenyls and organochlorine pesticides in serum/plasma by solid phase micro-extraction in headspace and gas chromatography coupled with electron capture detector (HS-SPME- GC-ECD). After determining the level of endocrine disrupting chemicals in each sample it was observed that the organochlorine pesticides were present in different concentrations, the combined value of these values ranging from 35,24 ng/ml in sample 5 to 60,87 ng/ml. Polychlorinated biphenyls were seldom identified, sample number 3 being PCB 28 positive and sample number 1, 2 and 3 to PCB 153. Heavy metals were identified in all the samples collected, with broad ranging results. These results, correlated with other epidemiological studies suggest without a doubt that these endocrine disruptors will also be present in a wide range of animal by-products. Furthermore, broader studies are of utmost importance for fully understanding the risk to human and animal health. en_US
dc.language.iso en en_US
dc.publisher ”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject MW-ICP-AES en_US
dc.subject HS-SPME-GC-ECD en_US
dc.subject disruptors en_US
dc.subject serum en_US
dc.title Nanoscale level determination methods of endocrine disruptors in animal fluids, and their effect upon the female genital tract en_US
dc.type Article en_US
dc.author.affiliation Vlad Ilaș, Adrian Henegariu, Mihai Cenariu, Emoke Pall, Ioan Groza, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania
dc.publicationName Lucrări Științifice USAMV - Iași, Seria Medicină Veterinară
dc.volume 59
dc.issue 4
dc.publicationDate 2016
dc.startingPage 458
dc.endingPage 462
dc.identifier.eissn 2393-4603


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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)