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The antibacterial activity and synergies between morusin and some antibiotics against MRSA strains – preliminary study

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dc.contributor.author Rîmbu, Cristina-Mihaela
dc.contributor.author Horhogea, Cristina
dc.contributor.author Aelenei, Petruța
dc.contributor.author Guguianu, Eleonora
dc.contributor.author Carp Cărare, Cătălin
dc.contributor.author Crețu, Carmen
dc.contributor.author Floriștean, Viorel-Cezar
dc.contributor.author Grecu, Mariana
dc.contributor.author Dimitriu, Gabriel
dc.contributor.author Miron, Anca
dc.date.accessioned 2021-05-07T05:07:26Z
dc.date.available 2021-05-07T05:07:26Z
dc.date.issued 2017
dc.identifier.citation Rîmbu, Cristina, Cristina Horhogea, Petruța Aelenei, Eleonora Guguianu, Catalin Carp-Cărare, Carmen Crețu, Viorel Floriștean, Mariana Grecu, Gabriel Dimitriu, Anca Miron. 2017. "The antibacterial activity and synergies between morusin and some antibiotics against MRSA strains – preliminary study". Lucrări Științifice USAMV - Iași Seria Medicină Veterinară 60 (1): 39-47. en_US
dc.identifier.uri https://repository.uaiasi.ro/xmlui/handle/20.500.12811/1111
dc.description.abstract Mulberry (Morus alba L., Moraceae) is one of the most valuable and rich in phytochemicals plant. Morusin is a prenylated flavonoid present in mulberry roots and leaves. The in vitro antibacterial activity of morusin and its interactions with conventional antibiotics (oxacillin, amoxicillin and gentamicin) were evaluated against four methicillin resistant Staphylococcus aureus clinical isolates (MRSA T1 – T4) with resistance to oxacillin and cefoxitin which had been isolated from dogs with various pathologies. Minimum inhibitory concentrations (MICs) were determined by the microdilution method. The interactions were assessed by the chequerboard method - with interpretation through fractional inhibitory concentration index (FICI) and isobologram analysis. The interactions were confirmed by the time-kill assay. MICs varied between 3.125 and 6.25μg/mL for morusin alone against all four MRSA clinical isolates. Chequerboard method showed synergies for the combinations: morusin – oxacillin (FICI=0.024 - 0.27), morusin – amoxicillin (FICI=0.024 - 0.27) and morusin - gentamicin (FICI=0.05 - 0.12) against all four tested isolates. Time-kill assay determined synergies for the following combinations: morusin – oxacillin against MRSA T1, morusin – amoxicillin against MRSA T2 and morusin - gentamicin against all four isolates. Our preliminary study evaluated the antibacterial activity of morusin and its ability to act synergistically with antibiotics; these results suggest that morusin might be a promising strategy to overcome antibiotic resistence. en_US
dc.language.iso en en_US
dc.publisher ”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject bacterial resistance en_US
dc.subject chequerboard en_US
dc.subject morusin en_US
dc.subject synergy en_US
dc.subject time-kill assay en_US
dc.title The antibacterial activity and synergies between morusin and some antibiotics against MRSA strains – preliminary study en_US
dc.type Article en_US
dc.author.affiliation Cristina Rîmbu, Cristina Horhogea, Eleonora Guguianu, Catalin Carp-Cărare, Carmen Crețu, Viorel Floriștean, Microbiology-Immunology Laboratory, Department of Public Health, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Ion Ionescu de la Brad Iași, Romania
dc.author.affiliation Petruța Aelenei, Anca Miron, Department of Pharmacognosy, Faculty of Pharmacy, University of Medicine and Pharmacy Grigore T. Popa Iasi, România
dc.author.affiliation Mariana Grecu, Department of Pharmacology Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Ion Ionescu de la Brad Iași, Romania
dc.author.affiliation Gabriel Dimitriu, Department of Preventive Medicine and Interdisciplinary, Discipline Medical Informatics and Biostatistics, Faculty of Medicine, University of Medicine and Pharmacy Grigore T. Popa, Iasi
dc.publicationName Lucrări Științifice USAMV - Iași, Seria Medicină Veterinară
dc.volume 60
dc.issue 1
dc.publicationDate 2017
dc.startingPage 39
dc.endingPage 47
dc.identifier.eissn 2393-4603


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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)